This study presents a faunal list of Copepoda Calanoida in the Gulf of Gabès (Tunisia). A total of 52 species belonging to15 families were reported in this study area, including 11 species belonging to family Acartiidae. Due to the geographical position of Gulf of Gabès in the middle of the Mediterranean Sea, the Tunisian Calanoida fauna in this area can be considered as a mixture of non-indigenous species mainly from Atlantic, cosmopolitan species, and endemic species.